RHBDF2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-RHBDF2 Antibody (N-term) at 1:2000 dilution + Mouse lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RHBDF2 Antibody (N-term) at 1:1000 dilution Lane 1: RPMI 8226 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: HT-1080 whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-RHBDF2 Antibody (N-term) at 1:2000 dilution + HACAT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
RHBDF2 Antibody (N-term) (Cat. #AP13588a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHBDF2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q6PJF5
Other Accession	NP_001005498.2, NP_078875.4
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	96686 Da
Antigen Region	80-109 aa

Additional Information
Gene ID	79651
Other Names	Inactive rhomboid protein 2, iRhom2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6, RHBDF2, IRHOM2, RHBDL5, RHBDL6
Target/Specificity	This RHBDF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human RHBDF2.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RHBDF2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RHBDF2
Synonyms	IRHOM2, RHBDL5, RHBDL6
Function	Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q80WQ6}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q80WQ6}. Cell membrane
Tissue Location	Found in the epidermis and esophageal epithelium.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.