癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ERAS Antibody (N-term) (A28)
Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q7Z444 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 25287 Da |
| Antigen Region | 20-50 aa |
| Gene ID | 3266 |
|---|---|
| Other Names | GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP |
| Target/Specificity | This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20~50 amino acids from the N-terminal region of human ERAS. |
| Dilution | IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ERAS Antibody (N-term) (A28) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ERAS |
|---|---|
| Synonyms | HRAS2, HRASP |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity). |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
REFERENCES
Kameda,T., Stem Cells 23 (10), 1535-1540 (2005)
Takahashi,K., Nature 423 (6939), 541-545 (2003)
Miyoshi,J., Nucleic Acids Res. 12 (4), 1821-1828 (1984)
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