Anti-ERAS Antibody

Western blot analysis of ERAS expression in mouse liver (A), mouse testis (B), rat liver (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)
Immunofluorescent analysis of ERAS staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q7Z444
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	25287 Da

Additional Information
Gene ID	3266
Other Names	HRAS2; HRASP; GTPase ERas; E-Ras; Embryonic stem cell-expressed Ras
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ERAS. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ERAS
Synonyms	HRAS2, HRASP
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity).
Cellular Location	Cell membrane; Lipid-anchor; Cytoplasmic side

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.