癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DRD2 Antibody (C-term) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P14416 |
| Other Accession | NP_057658.2, NP_000786.1 |
| Reactivity | Human, Mouse |
| Predicted | Rat, Bovine, Canine, Rabbit |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 50619 Da |
| Antigen Region | 307-336 aa |
| Gene ID | 1813 |
|---|---|
| Other Names | D(2) dopamine receptor, Dopamine D2 receptor, DRD2 |
| Target/Specificity | This DRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human DRD2. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DRD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DRD2 {ECO:0000303|PubMed:15389757, ECO:0000312|HGNC:HGNC:3023} |
|---|---|
| Function | G protein-coupled receptor for dopamine, a catecholamine neurotransmitter hormone that functions as the brain's 'reward chemical', driving motivation, reinforcement learning and pleasure (PubMed:21645528, PubMed:32103023, PubMed:32528175, PubMed:33353947, PubMed:33571431, PubMed:37221270, PubMed:39103320). Dopamine binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:32103023, PubMed:32528175, PubMed:33353947, PubMed:33571431, PubMed:37221270). Dopamine receptors can be classified in two categories: (1) DRD2 (together with DRD3 and DRD4) is a D2-type receptor and is coupled to G(i)/G(o) G proteins, mediating inhibition of adenylate cyclase activity, while D1-type receptors (DRD1 and DRD5) are coupled to G(s) G proteins and promote activation of adenylate cyclase activity (PubMed:32103023, PubMed:32528175, PubMed:33353947, PubMed:33571431, PubMed:37221270). In the central nervous system, DRD2 is mainly coupled to G(o)/GNAO1 (By similarity). D1- and D2-type dopamine receptors have antagonistic effects, particularly in the brain's striatum, where they govern voluntary movement and reward: D1-type receptors generally stimulate neural activity and promotes action, while D2-type receptors tend to inhibit neural activity and suppress movement (By similarity). DRD1 and DRD2 are the most abundant dopamine receptors in the central nervous system, especially in the basal ganglia and the prefrontal cortex (PubMed:33571431). DRD2 receptor plays a key role in risky decision-making by modulating reward sensitivity and executive control; DRD2 activation in nucleus accumbens cells leading to increased risk- taking and impulsivity (By similarity). DRD2 can also couple with G(q) by forming a heteromer with the ghrelin receptor GHSR in neurons independently of ghrelin (By similarity). Following DRD2 activation by dopamine in lumbosacral defecation centers, GHSR acts as a coreceptor promoting DRD2 coupling to G(q) at the expense of G(i)/G(o), regulating defecation (By similarity). In addition to dopamine, also acts as a receptor for tryptophan-derived metabolites in intestinal epithelial cells: activation by tryptophan metabolites produced by the gut microbiota initiates a phospholipase-C-dependent signaling that promotes colonization resistance by pathogens by controlling actin cytoskeletal organization in the gut epithelium (By similarity). Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5 (By similarity). Also acts as a receptor for D-lysergic (LSD) psychedelic drug (PubMed:40683247). |
| Cellular Location | Postsynaptic cell membrane {ECO:0000250|UniProtKB:P61169}; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Mainly active on postsynaptic membrane (By similarity). Specifically localizes to special plasma membrane microcompartments (PubMed:23493394) {ECO:0000250|UniProtKB:P61169, ECO:0000269|PubMed:23493394} |
| Tissue Location | [Isoform 1]: Expressed in the anterior pituitary gland. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing.
REFERENCES
Verma, V., et al. J. Biol. Chem. 285(45):35092-35103(2010)
Borroto-Escuela, D.O., et al. Biochem. Biophys. Res. Commun. 401(4):605-610(2010)
Stelzel, C., et al. J. Neurosci. 30(42):14205-14212(2010)
Huang, H.Y., et al. J. Formos. Med. Assoc. 109(10):736-739(2010)
Itokawa, M., et al. J. Pharmacol. Sci. 114(1):1-5(2010)
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