KLHL3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application ![]()
| WB, E |
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Primary Accession | Q9UH77 |
Other Accession | F1MBP6, NP_059111.2 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 64970 Da |
Antigen Region | 207-234 aa |
Gene ID | 26249 |
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Other Names | Kelch-like protein 3, KLHL3, KIAA1129 |
Target/Specificity | This KLHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-234 amino acids from the Central region of human KLHL3. |
Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | KLHL3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KLHL3 {ECO:0000303|PubMed:10843806, ECO:0000312|HGNC:HGNC:6354} |
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Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na- Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3 (PubMed:35179207). The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation (By similarity). |
Cellular Location | Cytoplasm, cytosol. Cytoplasm, cytoskeleton |
Tissue Location | Widely expressed.. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
REFERENCES
Schmutz, J., et al. Nature 431(7006):268-274(2004)
Yeo, A., et al. J. Neurochem. 76(6):1688-1700(2001)
Lai, F., et al. Genomics 66(1):65-75(2000)
Mizutani, A., et al. J. Biol. Chem. 275(13):9823-9831(2000)

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