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>   首页   >   产品   >   一抗   >   细胞生物学   >   KLHL3 Antibody (Center)   

KLHL3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - KLHL3 Antibody (Center) AP17126c
    Anti-KLHL3 Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9UH77
Other Accession F1MBP6, NP_059111.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 64970 Da
Antigen Region 207-234 aa
Additional Information
Gene ID 26249
Other Names Kelch-like protein 3, KLHL3, KIAA1129
Target/Specificity This KLHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-234 amino acids from the Central region of human KLHL3.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLHL3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLHL3 {ECO:0000303|PubMed:10843806, ECO:0000312|HGNC:HGNC:6354}
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na- Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3 (PubMed:35179207). The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation (By similarity).
Cellular Location Cytoplasm, cytosol. Cytoplasm, cytoskeleton
Tissue Location Widely expressed..
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

REFERENCES

Schmutz, J., et al. Nature 431(7006):268-274(2004)
Yeo, A., et al. J. Neurochem. 76(6):1688-1700(2001)
Lai, F., et al. Genomics 66(1):65-75(2000)
Mizutani, A., et al. J. Biol. Chem. 275(13):9823-9831(2000)

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