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>   首页   >   产品   >   一抗   >   细胞生物学   >   POLA2 Antibody (Center)   

POLA2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - POLA2 Antibody (Center) AP17301c
    POLA2 Antibody (Center) (Cat. #AP17301c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the POLA2 antibody detected the POLA2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q14181
Other Accession O89043, Q58D13, NP_002680.2
Reactivity Human
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 65948 Da
Antigen Region 212-240 aa
Additional Information
Gene ID 23649
Other Names DNA polymerase alpha subunit B, DNA polymerase alpha 70 kDa subunit, POLA2
Target/Specificity This POLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the Central region of human POLA2.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLA2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLA2
Function Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery (By similarity).

REFERENCES

Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Stelzl, U., et al. Cell 122(6):957-968(2005)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

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