癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DNA polymerase subunit II Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q14181 |
| Other Accession | Q14181 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 65948 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human POLA2 |
| Epitope Specificity | 101-200/598 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the DNA polymerase alpha subunit B family. |
| SUBUNIT | DNA polymerase alpha:primase is a four subunit enzymecomplex, which is assembled throughout the cell cycle, and consistsof the two DNA polymerase subunits A and B, and the DNA primaselarge and small subunits. Subunit B binds to subunit A. |
| Post-translational modifications | Phosphorylated in a cell cycle-dependent manner, in G2/M phase. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery. |
| Gene ID | 23649 |
|---|---|
| Other Names | DPOA2_HUMAN; POLA2; DNA polymerase alpha 70 kDa subunit; DNA polymerase alpha 2, accessory subunit; polymerase (DNA directed), alpha 2 (70kD subunit); polymerase (DNA) alpha 2, accessory subunit; DNA polymerase alpha subunit B |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | POLA2 |
|---|---|
| Function | Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis (PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerizing short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). |
| Cellular Location | Nucleus. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP97392
