癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CRB3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BUF7 |
| Other Accession | Q8QZT4, NP_631900.1, NP_777377.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 12854 Da |
| Antigen Region | 67-95 aa |
| Gene ID | 92359 |
|---|---|
| Other Names | Protein crumbs homolog 3, CRB3 |
| Target/Specificity | This CRB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the Central region of human CRB3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CRB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CRB3 (HGNC:20237) |
|---|---|
| Function | Involved in the establishment of cell polarity in mammalian epithelial cells (PubMed:12771187, PubMed:14718572, PubMed:23439680). Regulates the morphogenesis of tight junctions (PubMed:12771187, PubMed:14718572). Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity). |
| Cellular Location | Apical cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells. |
| Tissue Location | Preferentially expressed in epithelial tissues (PubMed:14718572). Expressed at high levels in lung, kidney, and colon (PubMed:12527193, PubMed:14718572). Expressed at high levels in retina, colon and mammary glands (PubMed:12527193). Moderately expressed in liver, spleen, pancreas and prostate (PubMed:12527193). Moderately to weakly expressed in the placenta (PubMed:12527193, PubMed:14718572) Weakly expressed in skeletal muscle and small intestine (PubMed:14718572). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a member of the Crumbs family of proteins. This protein may play a role in epithelial cell polarity and is associated with tight junctions at the apical surface of epithelial cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
REFERENCES
Pardossi-Piquard, R., et al. Biochemistry 46(48):13704-13710(2007)
Fan, S., et al. J. Cell Biol. 178(3):387-398(2007)
Fogg, V.C., et al. J. Cell. Sci. 118 (PT 13), 2859-2869 (2005) :
Lemmers, C., et al. Mol. Biol. Cell 15(3):1324-1333(2004)
Roh, M.H., et al. J. Cell. Sci. 116 (PT 14), 2895-2906 (2003) :
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