OLFM3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-OLFM3 Antibody (N-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Y79 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
OLFM3 Antibody (N-term) (Cat. #AP20399a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the OLFM3 antibody detected the OLFM3 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q96PB7
Other Accession	P63057, P63056
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	54930 Da
Antigen Region	114-142 aa

Additional Information
Gene ID	118427
Other Names	Noelin-3, Olfactomedin-3, Optimedin, OLFM3, NOE3
Target/Specificity	This OLFM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-142 amino acids from the N-terminal region of human OLFM3.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	OLFM3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	OLFM3
Synonyms	NOE3
Cellular Location	Secreted. Synapse.
Tissue Location	In the eye, expressed in trabecular meshwork and neural retina; in non-ocular tissues, expressed in brain and lung

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.