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PDCL Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - PDCL Antibody (N-term) AP20562a
    Immunohistochemical analysis of paraffin-embedded H.kidney section using PDCL Antibody (N-term)(Cat#AP20562a). AP20562a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - PDCL Antibody (N-term) AP20562a
    Western blot analysis of lysates from Hela, Y79 cell line (from left to right), using PDCL Antibody (N-term)(Cat. #AP20562a). AP20562a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q13371
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34282 Da
Additional Information
Gene ID 5082
Other Names Phosducin-like protein, PHLP, PDCL, PHLOP1, PhLP1
Target/Specificity This PDCL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PDCL.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDCL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDCL
Synonyms PHLOP1, PhLP1
Function Acts as a positive regulator of hedgehog signaling and regulates ciliary function. [Isoform 2]: Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
Cellular Location Cell projection, cilium {ECO:0000250|UniProtKB:Q9DBX2}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Isoform 1: Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.

REFERENCES

Lazarov M.E.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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