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EEF1B2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - EEF1B2 Antibody (Center) AP20573a
    Flow cytometric analysis of Hela cells using EEF1B2 Antibody (Center)(green, Cat#AP20573a) compared to an isotype control of rabbit IgG(blue). AP20573a was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - EEF1B2 Antibody (Center) AP20573a
    Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody (Center)(Cat#AP20573a). AP20573a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - EEF1B2 Antibody (Center) AP20573a
    Fluorescent image of Hela cells stained with EEF1B2 Antibody (Center)(Cat#AP20573a). AP20573a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 1 - EEF1B2 Antibody (Center) AP20573a
    Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody (Center)(Cat. #AP20573a). AP20573a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, FC, E
Primary Accession P24534
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 24764 Da
Additional Information
Gene ID 1933
Other Names Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
Target/Specificity This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:25
FC~~1:25
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1B2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1B2
Synonyms EEF1B, EF1B
Function Catalytic subunit of the guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) of the eukaryotic elongation factor 1 complex (eEF1) (By similarity). Stimulates the exchange of GDP for GTP on elongation factor 1A (eEF1A), probably by displacing GDP from the nucleotide binding pocket in eEF1A (By similarity).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

REFERENCES

Sanders J.,et al.Nucleic Acids Res. 19:4551-4551(1991).
von der Kammer H.,et al.Biochem. Biophys. Res. Commun. 177:312-317(1991).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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