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POU5F1B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - POU5F1B Antibody (N-term) AP21021a
    Western blot analysis of lysate from human skeletal muscle tissue lysate, using POU5F1B Antibody (N-term)(Cat. #AP21021a). AP21021a was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q06416
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38588 Da
Additional Information
Gene ID 5462
Other Names Putative POU domain, class 5, transcription factor 1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like, POU5F1B
Target/Specificity This POU5F1B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the N-terminal region of human POU5F1B.
Dilution WB~~1:500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU5F1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU5F1B
Function Shows weak transcriptional activator activity.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:18949397} Cytoplasm
Tissue Location Detected in epithelial cells of the prostate (at protein level) (PubMed:20017164). Detected at the mRNA level in several cancer tissues (breast, uterine cervix, lung, thyroid gland, esophagus, colon, urinary bladder, and glioma) (PubMed:16229821, PubMed:21341266)
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Shows weak transcriptional activator activity.

REFERENCES

Takeda J.,et al.Nucleic Acids Res. 20:4613-4620(1992).
Marques A.C.,et al.PLoS Biol. 3:E357-E357(2005).
van Roozendaal K.E.P.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Zhao F.-Q.,et al.Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
Zhao S.,et al.Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.

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