(Mouse) Scx Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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AP21125a staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Western blot analysis of lysate from mouse lung tissue lysate, using Scx Antibody (N-term)(Cat. #AP21125a). AP21125a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q64124
Reactivity	Human, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	22239 Da

Additional Information
Gene ID	20289
Other Names	Basic helix-loop-helix transcription factor scleraxis, Scx
Target/Specificity	This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	(Mouse) Scx Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	Scx
Function	Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00981}.
Tissue Location	Expressed in mesenchymal precursors of cartilage and in connective tissue. Highly expressed in tendons in the limb, tongue and diaphragm and in cartilage of the bronchi

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.