(Mouse) Eras Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-Eras Antibody (C-term) at 1:2000 dilution Lane 1: mouse heart lysates Lane 2: rat heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Eras Antibody (C-term)at 1:2000 dilution + mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q7TN89
Reactivity	Rat, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	24321 Da

Additional Information
Gene ID	353283
Other Names	GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras
Target/Specificity	This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 202-236 amino acids from the C-terminal region of Mouse Eras.
Dilution	WB~~1:2000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	(Mouse) Eras Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Eras
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Cellular Location	Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location	Expressed in several undifferentiated mouse embryonic stem cell lines.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.