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NSFL1C Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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Overlay histogram showing A431 cells stained with AP22236c(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
AP22236c staining NSFL1C in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-NSFL1C Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q9UNZ2
Other Accession	Q3SZC4, Q9CZ44, Q5RBG3, O35987
Reactivity	Human, Rat, Mouse
Predicted	Bovine, Mouse, Rat
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	40573 Da

Additional Information
Gene ID	55968
Other Names	NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1C, UBXN2C
Target/Specificity	This NSFL1C antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 214-248 amino acids from the Central region of human NSFL1C.
Dilution	WB~~1:2000 IHC-P~~1:100~500 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	NSFL1C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NSFL1C
Synonyms	UBXN2C
Function	Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP- mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed:15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:O35987}. Golgi apparatus, Golgi stack {ECO:0000250\|UniProtKB:O35987}. Chromosome {ECO:0000250\|UniProtKB:O35987}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250\|UniProtKB:O35987} Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks Localizes to centrosome during mitotic prophase and metaphase {ECO:0000250\|UniProtKB:O35987}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro).

REFERENCES

Yue P.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).

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¥ 1,250.00

Cat# AP22236c

Size:

50 µl

100 µl

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Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

NSFL1C Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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