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NRAS Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - NRAS Antibody AP22295a
    AP22295a staining NRAS in human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - NRAS Antibody AP22295a
    Anti-NRAS Antibody at 1:2000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P01111
Other Accession Q2MJK3
Reactivity Human, Rat, Mouse
Predicted Pig
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 21229 Da
Additional Information
Gene ID 4893
Other Names GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
Target/Specificity This NRAS antibody is generated from a rabbit immunized with arecombinant protein from the human region of human NRAS.
Dilution WB~~1:2000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

REFERENCES

Taparowsky E.,et al.Cell 34:581-586(1983).
Hall A.,et al.Nucleic Acids Res. 13:5255-5268(1985).
Brown R.,et al.EMBO J. 3:1321-1326(1984).
Yuasa Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 81:3670-3674(1984).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.

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