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PDHE1A(S232) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PDHE1A(S232) Antibody AP22387a
    All lanes: Anti-PDHE1A(S232) Antibody at 1:2000 dilution + PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 43 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - PDHE1A(S232) Antibody AP22387a
    All lanes : Anti-PDHE1A(S232) Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: RD whole cell lysate Lane 4: Mouse brain whole lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P08559
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 43296 Da
Additional Information
Gene ID 5160
Other Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, 1.2.4.1, PDHE1-A type I, PDHA1, PHE1A
Target/Specificity This PDHE1A(S232) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 206-237 amino acids from the human region of human PDHE1A(S232).
Dilution WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDHE1A(S232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHA1
Synonyms PHE1A
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

REFERENCES

Koike K.,et al.Gene 93:307-311(1990).
Ho L.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:5330-5334(1989).
Huh T.L.,et al.Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases.
Dahl H.-H.M.,et al.J. Biol. Chem. 262:7398-7403(1987).
Maragos C.,et al.J. Biol. Chem. 264:12294-12298(1989).

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