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Phospho-PDHE1A(S232) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Phospho-PDHE1A(S232) Antibody AP3758A
    Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
  • 1 - Phospho-PDHE1A(S232) Antibody AP3758A
    Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
  • 1 - Phospho-PDHE1A(S232) Antibody AP3758A
    Western blot analysis of lysates from 293T-17 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
  • 6 - Phospho-PDHE1A(S232) Antibody AP3758A
    Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab (Cat. #AP3758a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, DB, E
Primary Accession P08559
Other Accession P26284, P29804, P35486, Q8HXW9, A7MB35, NP_000275.1
Reactivity Human
Predicted Bovine, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43296 Da
Additional Information
Gene ID 5160
Other Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A
Target/Specificity This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.
Dilution WB~~1:5000
DB~~1:500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-PDHE1A(S232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHA1
Synonyms PHE1A
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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