Phospho-PDHE1A(S232) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
Western blot analysis of lysates from 293T-17 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A(S232) Antibody(upper) or Tubulin (lower).
Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab (Cat. #AP3758a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, DB, E
Primary Accession	P08559
Other Accession	P26284, P29804, P35486, Q8HXW9, A7MB35, NP_000275.1
Reactivity	Human
Predicted	Bovine, Monkey, Mouse, Pig, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	43296 Da

Additional Information
Gene ID	5160
Other Names	Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A
Target/Specificity	This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.
Dilution	WB~~1:5000 DB~~1:500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Phospho-PDHE1A(S232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PDHA1
Synonyms	PHE1A
Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location	Mitochondrion matrix.
Tissue Location	Ubiquitous.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.