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PHPT1 Antibody( C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-PHPT1 Antibody( C-term) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: 293 whole cell lysate Lane 4: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 13. 8 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody (N-term) (Cat.#AP2721b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q9NRX4
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	13833 Da
Antigen Region	88-117 aa

Additional Information
Gene ID	29085
Other Names	14 kDa phosphohistidine phosphatase, 313-, Phosphohistidine phosphatase 1, Protein janus-A homolog, PHPT1, PHP14
Target/Specificity	This PHPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the C-terminal region of human PHPT1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PHPT1 Antibody( C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PHPT1
Synonyms	PHP14
Function	Exhibits phosphohistidine phosphatase activity.
Cellular Location	Cytoplasm.
Tissue Location	Expressed abundantly in heart and skeletal muscle.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek etal., 2002 [PubMed 12383260]).[supplied by OMIM].

REFERENCES

Lim,J., Cell 125 (4), 801-814 (2006)
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Ma,R.,Biochem. Biophys. Res. Commun. 337 (3), 887-891 (2005)

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¥ 1,250.00

Cat# AP2721b

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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询价

PHPT1 Antibody( C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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