PCNA Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application ![]()
| WB, IHC-P, IF, E |
---|---|
Primary Accession | P12004 |
Other Accession | P04961, P61258, P57761, Q3ZBW4 |
Reactivity | Human |
Predicted | Bovine, Hamster, Monkey, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 28769 Da |
Gene ID | 5111 |
---|---|
Other Names | Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA |
Target/Specificity | This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA. |
Dilution | WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PCNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PCNA |
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Function | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'- phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737). |
Cellular Location | Nucleus Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |

Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
REFERENCES
Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

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