癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PCNA Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- 文献引用 : 6
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P12004 |
| Other Accession | P04961, P61258, P57761, Q3ZBW4 |
| Reactivity | Human |
| Predicted | Bovine, Hamster, Monkey, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 28769 Da |
| Gene ID | 5111 |
|---|---|
| Other Names | Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA |
| Target/Specificity | This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 IF~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PCNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PCNA |
|---|---|
| Function | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'- phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737). |
| Cellular Location | Nucleus Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
REFERENCES
Wang,Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga,G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya,N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)
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