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RFC3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RFC3 Antibody (Center) AP2860c
    Western blot analysis of anti-RFC3 Antibody (Center) (Cat.#AP2860c) in 293 cell line lysates (35ug/lane). RFC3 (arrow) was detected using the purified Pab.
  • 4 - RFC3 Antibody (Center) AP2860c
    RFC3 Antibody (Center) (Cat. #AP2860c) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P40938
Other Accession Q8R323, Q2TBV1
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40556 Da
Antigen Region 121-150 aa
Additional Information
Gene ID 5983
Other Names Replication factor C subunit 3, Activator 1 38 kDa subunit, A1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3
Target/Specificity This RFC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human RFC3.
Dilution WB~~1:1000
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFC3
Function Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.

REFERENCES

Rauen M., Burtelow M.A.J. Biol. Chem. 275:29767-29771(2000)

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