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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-CRK(S41) Antibody   

Phospho-CRK(S41) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-CRK(S41) Antibody AP3304a
    Dot blot analysis of Phospho-CRK-S41 polyclonal antibody (Cat# AP3304a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession P46108
Other Accession Q63768, Q64010, Q96GA9
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33831 Da
Additional Information
Gene ID 1398
Other Names Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK
Target/Specificity This CRK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S41 of human CRK.
Dilution DB~~1:500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CRK(S41) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRK
Function Involved in cell branching and adhesion mediated by BCAR1- CRK-RAPGEF1 signaling and activation of RAP1.
Cellular Location Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

REFERENCES

Bougneres, L., et al., J. Cell Biol. 166(2):225-235 (2004).
Stoletov, K.V., et al., Exp. Cell Res. 295(1):258-268 (2004).
Miller, C.T., et al., Oncogene 22(39):7950-7957 (2003).
Sun, J., et al., J. Biol. Chem. 278(35):32794-32800 (2003).
Zhang, X.A., et al., J. Biol. Chem. 278(29):27319-27328 (2003).

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