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RDH10 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RDH10 Antibody (Center) AP4738c
    RDH10 Antibody (Center) (Cat. #AP4738c) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).
  • 14 - RDH10 Antibody (Center) AP4738c
    RDH10 Antibody (Center) (Cat. #AP4738c) immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RDH10 Antibody (Center) AP4738c
    RDH10 Antibody (Center) (Cat. #AP4738c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession Q8IZV5
Other Accession Q80ZF7, Q8VCH7, Q8HZT6
Reactivity Human, Rat, Mouse
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38087 Da
Antigen Region 106-135 aa
Additional Information
Gene ID 157506
Other Names Retinol dehydrogenase 10, RDH10
Target/Specificity This RDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human RDH10.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRDH10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RDH10
Synonyms SDR16C4
Function Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol.
Cellular Location Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

RDH10 generates all-trans retinal from all-trans retinol and may plan an important role in the photic visual cycle. All-trans retinal is isomerized to 11-cis retinal by the retinal G protein-coupled receptor (RGR; MIM 600342) when the retinal pigment epithelium (RPE) is illuminated.

REFERENCES

Bankovic, J., et al. Lung Cancer 67(2):151-159(2010)
Takahashi, Y., et al. Biochem. J. 419(1):113-122(2009)
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009)

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