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Anti-APLP1 Antibody

Western blot analysis of APLP1 expression in HEK293T (A), H1688 (B), H1792 (C), mouse kidney (D), mouse heart (E), rat heart (F) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 97 kD)
Immunofluorescent analysis of APLP1 staining in PCCL cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P51693
Reactivity	Human, Mouse, Rat, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	72176 Da

Additional Information
Gene ID	333
Other Names	Amyloid-like protein 1; APLP; APLP-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APLP1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	APLP1
Function	May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Paliga K.,et al.Eur. J. Biochem. 250:354-363(1997).
Lenkkeri U.,et al.Hum. Genet. 102:192-196(1998).
Grimwood J.,et al.Nature 428:529-535(2004).
Kim T.-W.,et al.Brain Res. Mol. Brain Res. 32:36-44(1995).
Bush A.I.,et al.J. Biol. Chem. 269:26618-26621(1994).

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¥ 1,250.00

Cat# AP50984

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Anti-APLP1 Antibody

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