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Anti-Cystatin B Antibody

Western blot analysis of Cystatin B expression in JAR (A), EOC20 (B), U87MG (C) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 11 kD)
Immunohistochemical analysis of Cystatin B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cystatin B staining in EOC20 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P04080
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	11140 Da

Additional Information
Gene ID	1476
Other Names	CST6; STFB; Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cystatin B. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CSTB
Synonyms	CST6, STFB
Function	This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Cellular Location	Cytoplasm. Nucleus

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Ritonja A.,et al.Biochem. Biophys. Res. Commun. 131:1187-1192(1985).
Pennacchio L.A.,et al.Science 271:1731-1734(1996).
Bhat K.S.,et al.Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases.
Hattori M.,et al.Nature 405:311-319(2000).
Riccio M.,et al.Exp. Cell Res. 262:84-94(2001).

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¥ 1,250.00

Cat# AP51123

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Anti-Cystatin B Antibody

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