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Anti-ELMO1 Antibody

Western blot analysis of ELMO1 expression in mouse liver (A), mouse brain (B), rat liver (C), rat brain (D) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)
Immunofluorescent analysis of ELMO1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q92556
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	83829 Da

Additional Information
Gene ID	9844
Other Names	KIAA0281; Engulfment and cell motility protein 1; Protein ced-12 homolog
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ELMO1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ELMO1
Synonyms	KIAA0281
Function	Regulates the activity of DOCK guanine nucleotide exchange factors (GEFs) (PubMed:11595183, PubMed:12134158, PubMed:32651375, PubMed:38857861). Acts in association with DOCK1, DOCK2, DOCK5 and CRK (PubMed:11595183, PubMed:12134158, PubMed:32651375, PubMed:38857861). Promotes RAC1 activation by DOCK5; the activity is stimulated by RHOG (PubMed:38857861). Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility (PubMed:11595183).
Cellular Location	Cytoplasm. Cell membrane. Note=Translocation to plasma membrane seems to be mediated by DOCK1 and CRK
Tissue Location	Widely expressed, with a higher expression in the spleen and placenta

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Gumienny T.L.,et al.Cell 107:27-41(2001).
Ohara O.,et al.DNA Res. 4:53-59(1997).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP51187

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Anti-ELMO1 Antibody

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