Anti-TEL Antibody

Western blot analysis of TEL expression in zebrafish (A) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 57 kD)
Immunohistochemical analysis of TEL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TEL staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P41212
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53000 Da

Additional Information
Gene ID	2120
Other Names	TEL; TEL1; Transcription factor ETV6; ETS translocation variant 6; ETS-related protein Tel1; Tel
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TEL. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ETV6
Synonyms	TEL, TEL1
Function	Transcriptional repressor; binds to the DNA sequence 5'- CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.
Cellular Location	Nucleus.
Tissue Location	Ubiquitous.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Golub T.R.,et al.Cell 77:307-316(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Baens M.,et al.Genome Res. 6:404-413(1996).
Mullighan C.G.,et al.Nature 446:758-764(2007).

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¥ 1,250.00

Cat# AP51198

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Anti-TEL Antibody

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