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Anti-FAST Kinase Antibody

Western blot analysis of FAST Kinase expression in HEK293T (A), H460 (B), mouse brain (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)
Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q14296
Reactivity	Human, Mouse, Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	61104 Da

Additional Information
Gene ID	10922
Other Names	Fas-activated serine/threonine kinase; FAST kinase
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FAST Kinase. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FASTK
Function	Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro- apoptotic form of the receptor.
Cellular Location	[Isoform 4]: Mitochondrion matrix. Note=Colocalizes with mitochondrial RNA granules.
Tissue Location	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Tian Q.,et al.J. Exp. Med. 182:865-874(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP51205

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Anti-FAST Kinase Antibody

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