癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
FPR1 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P21462 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 38446 Da |
| Gene ID | 2357 |
|---|---|
| Other Names | fMet-Leu-Phe receptor, fMLP receptor, N-formyl peptide receptor, FPR, N-formylpeptide chemoattractant receptor, FPR1 |
| Target/Specificity | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FPR1. The exact sequence is proprietary. |
| Dilution | WB~~1:1000 IHC-P~~1:50~200 |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
| Name | FPR1 {ECO:0000303|PubMed:25109685} |
|---|---|
| Function | Pattern recognition G-protein coupled receptor (PRR/GPCR) involved in innate recognition of N-formyl-methionyl peptides derived from invading microbes and host mitochondria as pathogen- and damage- associated molecular patterns (PAMPs and DAMPs). Functions as a sensor of PAMPs and DAMPs released upon microbial infection or tissue damage, triggering immune cell activation and chemotaxis to eliminate pathogens and restore tissue homeostasis (PubMed:24108355, PubMed:25605714, PubMed:35217703, PubMed:36064945). Peptide binding leads to conformational changes coupled to heterotrimeric G(i) protein signaling. Upon GDP to GTP conversion, G(i)-alpha subunit dissociates from G-beta and G-gamma subunits. Free G(i)-alpha subunit inhibits cyclic adenylate cyclase and cAMP synthesis whereas the G-beta and G- gamma dimer activates downstream phospholipase C-beta and phosphoinositide 3-kinase signaling cascades leading to Ca(2+) influx (PubMed:10514456, PubMed:15153520, PubMed:1712023, PubMed:25605714, PubMed:35217703, PubMed:36064945). Displays two affinity states for peptide agonists, low and high, likely accounting for selective signaling of myeloid cell functions at different phases of the inflammatory response. Subnanomolar concentrations of peptide agonists induce myeloid cell chemotaxis, whereas micromolar concentrations trigger degranulation and superoxide production (PubMed:2161213, PubMed:2176894, PubMed:24108355, PubMed:25605714). May recognize a myriad of bacterial signal peptides indicative of an evolutionary conserved detection mechanism in host defense against bacterial infection. Triggers bactericidal functions of neutrophils and phagocytes in response to N-formyl-Met-Leu-Phe (fMLF) which is part of the signal peptide sequences of hundreds distinct bacterial strains (PubMed:25605714). In the homeostatic wound healing response to tissue injury, senses 'necrotaxis' DAMP-type signals released in the form of mitochondria-derived N-formylated peptides and guides neutrophil trafficking toward necrotic cells within the injury site (By similarity). In the context of antitumor immunity, interacts with ANXA1 and guides dendritic cell positioning in close proximity to necrotic tumor cells, allowing for tumor-associated antigen uptake and cross- presentation to T cells (PubMed:24108355, PubMed:26516201). Receptor for TAFA4, mediates its effects on chemoattracting macrophages, promoting phagocytosis and increasing reactive oxygen species (ROS) release (PubMed:25109685). Receptor for cathepsin CTSG, leading to increased phagocyte chemotaxis (PubMed:15210802). Beyond canonical N- terminal formylated peptide agonists, can also be activated by C- terminal amidated peptides, which appear to all share a tripartite structure motif oriented around a carboxyl group (PubMed:24108355, PubMed:25605714). Differential signaling is also defined by receptor oligomerization state. Pro-resolving ligands, such as lipoxin A4 or ANXA1, induce the formation of FPR1:FPR2 heterodimers triggering proapoptotic JNK pathway in neutrophils (PubMed:24108355). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Note=Internalizes in presence of its ligands, fMLP, TAFA4 and CTSG. |
| Tissue Location | Monocytes (at protein level) (PubMed:25605714). Neutrophils. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
High affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
REFERENCES
Boulay F.,et al.Biochem. Biophys. Res. Commun. 168:1103-1109(1990).
Boulay F.,et al.Biochemistry 29:11123-11133(1990).
Murphy P.M.,et al.J. Biol. Chem. 266:12560-12567(1991).
Bao L.,et al.Genomics 13:437-440(1992).
Perez H.D.,et al.Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
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