DGKK Antibody (C-term) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of DGKK Antibody (C-term) (Cat. #AP5122b) in mouse lung tissue lysates (35ug/lane).DGKK (arrow) was detected using the purified Pab.
DGKK Antibody (C-term) (Cat. #AP5122b) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DGKK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, E
Primary Accession	Q5KSL6
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	141829 Da
Antigen Region	1232-1261 aa

Additional Information
Gene ID	139189
Other Names	Diacylglycerol kinase kappa, DAG kinase kappa, 142 kDa diacylglycerol kinase, Diglyceride kinase kappa, DGK-kappa, DGKK
Target/Specificity	This DGKK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1232-1261 amino acids from the C-terminal region of human DGKK.
Dilution	IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DGKK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DGKK (HGNC:32395)
Function	Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:16210324, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).
Cellular Location	Cell membrane; Peripheral membrane protein
Tissue Location	Expressed in testis, and to a lesser extent in placenta.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.