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Anti-MCM4 Antibody

Western blot analysis of MCM4 expression in HeLa (A) whole cell lysates. (Predicted band size: 96 kD; Observed band size: 110 kD)
Immunohistochemical analysis of MCM4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P33991
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	96558 Da

Additional Information
Gene ID	4173
Other Names	CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MCM4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MCM4 (HGNC:6947)
Synonyms	CDC21
Function	Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914).
Cellular Location	Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Musahl C.,et al.Eur. J. Biochem. 230:1096-1101(1995).
Connelly M.A.,et al.Genomics 47:71-83(1998).
Ladenburger E.M.,et al.Cytogenet. Cell Genet. 77:268-270(1997).
Hu B.,et al.Nucleic Acids Res. 21:5289-5293(1993).
Ishimi Y.,et al.J. Biol. Chem. 272:24508-24513(1997).

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¥ 1,250.00

Cat# AP51337

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Anti-MCM4 Antibody

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