癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Patched Antibody
- 产品详情
- 文献引用 : 1
- 实验流程
Application 
| WB, IHC |
|---|---|
| Primary Accession | Q13635 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 160545 Da |
| Gene ID | 5727 |
|---|---|
| Other Names | PTCH; Protein patched homolog 1; PTC; PTC1 |
| Target/Specificity | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Patched. The exact sequence is proprietary. |
| Dilution | WB~~WB (1/500 - 1/1000) IHC~~1:100~500 |
| Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PTCH1 {ECO:0000303|PubMed:31555730, ECO:0000312|HGNC:HGNC:9585} |
|---|---|
| Function | Negative regulator of the smoothened signaling pathway, which acts as a receptor for hedgehog (SHH, IHH and DHH) morphogens (PubMed:21537345, PubMed:29954986, PubMed:29995851, PubMed:30139912, PubMed:31127104, PubMed:31555730, PubMed:37611095, PubMed:40128518, PubMed:9811851). In absence of hedgehog, acts as an inhibitor of smoothened protein (SMO) by preventing SMO access to cholesterol: mechanistically, acts as a cholesterol transporter, which depeletes cholesterol from the outer leaflet of plasma membrane, thereby preventing cholesterol-binding to the extracellular CRD region of SMO (PubMed:31555730, PubMed:37611095). Hedgehog-binding inhibits PTCH1 cholesterol transporter activity, promoting SMO activation by cholesterol and transduction of the smoothened signaling pathway (PubMed:31548691). Molecular mechanism governing cholesterol transport are still unclear: may move cholesterol from the outer to the inner leaflet of the membrane in exchange for potassium or sodium ion export in the opposite direction (PubMed:37611095). Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis (PubMed:8658145). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell projection, cilium membrane {ECO:0000250|UniProtKB:Q61115}; Multi-pass membrane protein Note=Localizes to primary cilium and prevents cilium localization of SMO (PubMed:29954986, PubMed:29995851, PubMed:30139912, PubMed:31127104, PubMed:31555730). Delocalizes from primary cilium following hedgehog-binding (By similarity) {ECO:0000250|UniProtKB:Q61115, ECO:0000269|PubMed:29954986, ECO:0000269|PubMed:29995851, ECO:0000269|PubMed:30139912, ECO:0000269|PubMed:31127104, ECO:0000269|PubMed:31555730} |
| Tissue Location | In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Johnson R.L.,et al.Science 272:1668-1671(1996).
Hahn H.,et al.J. Biol. Chem. 271:12125-12128(1996).
Humphray S.J.,et al.Nature 429:369-374(2004).
Nagao K.,et al.Genomics 85:462-471(2005).
Shimokawa T.,et al.Oncogene 26:4889-4896(2007).
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