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Anti-RIT1 Antibody

Western blot analysis of RIT1 expression in Hela (A), mouse liver (B), mouse kidney (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)
Immunofluorescent analysis of RIT1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q92963
Reactivity	Human, Mouse, Pig, Bovine, Dog, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	25145 Da

Additional Information
Gene ID	6016
Other Names	RIBB; RIT; ROC1; GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RIT1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RIT1
Synonyms	RIBB, RIT, ROC1
Function	Plays a crucial role in coupling nerve growth factor (NGF) stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation (PubMed:15632082). Involved in ELK1 transactivation through the Ras- MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation (PubMed:23791108). Recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (PubMed:40934300).
Cellular Location	Cell membrane.
Tissue Location	Expressed in many tissues.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Lee C.H.J.,et al.J. Neurosci. 16:6784-6794(1996).
Wes P.D.,et al.EMBO J. 15:5839-5848(1996).
Kawasaki H.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Shao H.,et al.Arch. Biochem. Biophys. 371:207-219(1999).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP51476

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Anti-RIT1 Antibody

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