> 首页 > 产品 > 一抗 > 癌症 > Anti-SNAP25 Antibody

Anti-SNAP25 Antibody

Western blot analysis of SNAP25 expression in HeLa (A), SKNSH (B), RAW264.7 (C), PC12 (D), rat brain (E) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 25 kD)
Immunohistochemical analysis of SNAP25 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P60880
Reactivity	Human, Mouse, Rat, Zebrafish, Pig, Chicken, Bovine, Dog, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	23315 Da

Additional Information
Gene ID	6616
Other Names	SNAP; Synaptosomal-associated protein 25; SNAP-25; Super protein; SUP; Synaptosomal-associated 25 kDa protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SNAP25. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SNAP25
Synonyms	SNAP
Function	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Cellular Location	Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:P60879}. Cell membrane {ECO:0000250\|UniProtKB:P60881}; Lipid-anchor {ECO:0000250\|UniProtKB:P60879}. Synapse, synaptosome {ECO:0000250\|UniProtKB:P60879}. Photoreceptor inner segment {ECO:0000250\|UniProtKB:P60879}. Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). Colocalizes with PLCL1 at the cell membrane (By similarity). {ECO:0000250\|UniProtKB:P60879, ECO:0000250\|UniProtKB:P60881}
Tissue Location	Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Bark I.C.,et al.Gene 139:291-292(1994).
Zhao N.,et al.Gene 145:313-314(1994).
Jagadish M.N.,et al.Biochem. J. 317:945-954(1996).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP51530

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

reduce add

Availability:

询价

Anti-SNAP25 Antibody

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务