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Anti-Topoisomerase 2 alpha Antibody [KO/KD Validated]

Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), H446 (B) whole cell lysates. (Predicted band size: 174 kD; Observed band size: 190 kD)
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Topoisomerase 2 alpha staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of Topoisomerase 2 alpha expression in wild type (WT) and knockdown (KD) HeLa cell lysates.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P11388
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	174385 Da

Additional Information
Gene ID	7153
Other Names	TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Topoisomerase 2 alpha. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TOP2A
Synonyms	TOP2
Function	Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612, PubMed:27754753). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
Cellular Location	Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus
Tissue Location	Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Tsai-Pflugfelder M.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A.,et al.Cancer Res. 53:3591-3596(1993).
Lang A.J.,et al.Gene 221:255-266(1998).
Sng J.H.,et al.Biochim. Biophys. Acta 1444:395-406(1999).
Zody M.C.,et al.Nature 440:1045-1049(2006).

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Anti-Topoisomerase 2 alpha Antibody [KO/KD Validated]

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