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>   首页   >   产品   >   一抗   >   癌症   >   Topoisomerase 2 alpha Antibody   

Topoisomerase 2 alpha Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Topoisomerase 2 alpha Antibody AP51577
    Anti-Topoisomerase 2 alpha Antibodyat 1:1000 dilution + Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 174 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P11388
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 174385 Da
Additional Information
Gene ID 7153
Other Names DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Target/Specificity KLH conjugated synthetic peptide derived from human Topoisomerase 2 alpha
Dilution WB~~ 1:1000
Format 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50%
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TOP2A
Synonyms TOP2
Function Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
Cellular Location Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus
Tissue Location Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056)
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

REFERENCES

Tsai-Pflugfelder M.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A.,et al.Cancer Res. 53:3591-3596(1993).
Lang A.J.,et al.Gene 221:255-266(1998).
Sng J.H.,et al.Biochim. Biophys. Acta 1444:395-406(1999).
Zody M.C.,et al.Nature 440:1045-1049(2006).

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