癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
TOP2A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 1
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P11388 |
| Other Accession | P41516, Q01320, P41515 |
| Reactivity | Human, Rat, Mouse |
| Predicted | Hamster, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 174385 Da |
| Antigen Region | 1167-1196 aa |
| Gene ID | 7153 |
|---|---|
| Other Names | DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2 |
| Target/Specificity | This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A. |
| Dilution | WB~~1:2000 IHC-P~~N/A IF~~1:50~200 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TOP2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TOP2A |
|---|---|
| Synonyms | TOP2 |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity). |
| Cellular Location | Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus |
| Tissue Location | Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
REFERENCES
Kawamura,R., et.al, J. Cell Biol. 188 (5), 653-663 (2010)
Alchanati,I., et.al, PLoS ONE 4 (12), E8104 (2009)
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