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Anti-GPR175 Antibody

Western blot analysis of GPR175 expression in Hela (A), DLD (B), H460 (C), A2780 (D), mouse brain (E), mouse heart (F) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)
Immunofluorescent analysis of GPR175 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q86W33
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	41053 Da

Additional Information
Gene ID	131601
Other Names	GPR175; TMEM227; Transmembrane protein adipocyte-associated 1; Integral membrane protein GPR175; Transmembrane protein 227
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR175. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TPRA1
Synonyms	GPR175, TMEM227
Cellular Location	Membrane; Multi-pass membrane protein.
Tissue Location	Ubiquitous, with higher levels in heart, placenta and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Saito T.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Yang H.,et al.Endocrinology 140:2859-2867(1999).

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¥ 1,250.00

Cat# AP51583

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100 µl

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Anti-GPR175 Antibody

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