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TPRA1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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Overlay histogram showing U-2OS cells stained with AP21977a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21977a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-TPRA1 Antibody (N-Term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q86W33
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	41053 Da

Additional Information
Gene ID	131601
Other Names	Transmembrane protein adipocyte-associated 1, Integral membrane protein GPR175, Transmembrane protein 227, TPRA1, GPR175, TMEM227
Target/Specificity	This TPRA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-47 amino acids from human TPRA1.
Dilution	WB~~1:2000 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TPRA1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	TPRA1
Synonyms	GPR175, TMEM227
Cellular Location	Membrane; Multi-pass membrane protein.
Tissue Location	Ubiquitous, with higher levels in heart, placenta and kidney.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Saito T.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Yang H.,et al.Endocrinology 140:2859-2867(1999).

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¥ 1,250.00

Cat# AP21977a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

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TPRA1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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