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Anti-Renin Receptor Antibody

Western blot analysis of Renin Receptor expression in THP1 (A), HEK293T (B), Hela (C), CT26 (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 48 kD)
Immunohistochemical analysis of Renin Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Renin Receptor staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	O75787
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	39008 Da

Additional Information
Gene ID	10159
Other Names	ATP6IP2; CAPER; ELDF10; Renin receptor; ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ER-localized type I transmembrane adaptor; Embryonic liver differentiation factor 10; N14F; Renin/prorenin receptor; Vacuolar ATP synthase membrane sector-associated protein M8-9; ATP6M8-9; V-ATPase M8.9 subunit
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Renin Receptor. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ATP6AP2 (HGNC:18305)
Function	Multifunctional protein which functions as a renin, prorenin cellular receptor and is involved in the assembly of the lysosomal proton-transporting V-type ATPase (V-ATPase) and the acidification of the endo-lysosomal system (PubMed:12045255, PubMed:29127204, PubMed:30374053, PubMed:32276428). May mediate renin-dependent cellular responses by activating ERK1 and ERK2 (PubMed:12045255). By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, may also play a role in the renin-angiotensin system (RAS) (PubMed:12045255). Through its function in V-type ATPase (v- ATPase) assembly and acidification of the lysosome it regulates protein degradation and may control different signaling pathways important for proper brain development, synapse morphology and synaptic transmission (By similarity).
Cellular Location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Lysosome membrane; Single- pass type I membrane protein. Cytoplasmic vesicle, autophagosome membrane {ECO:0000250\|UniProtKB:Q9CYN9}; Single-pass type I membrane protein. Cell projection, dendritic spine membrane {ECO:0000250\|UniProtKB:Q9CYN9}; Single-pass type I membrane protein. Cell projection, axon {ECO:0000250\|UniProtKB:Q9CYN9}. Endosome membrane {ECO:0000250\|UniProtKB:Q9CYN9}; Single-pass type I membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250\|UniProtKB:Q6AXS4}; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:Q6AXS4}; Single-pass type I membrane protein
Tissue Location	Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Nguyen G.,et al.J. Clin. Invest. 109:1417-1427(2002).
Wang J.,et al.Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
Hui R.T.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Otsuki T.,et al.DNA Res. 12:117-126(2005).

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Cat# AP51665

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Anti-Renin Receptor Antibody

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