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Anti-Dopamine Receptor D1 Antibody

Western blot analysis of Dopamine Receptor D1 expression in A549 (A), U2OS (B), mouse kidney (C), rat liver (D) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 60 kD)
Immunohistochemical analysis of Dopamine Receptor D1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Dopamine Receptor D1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P21728
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	49293 Da

Additional Information
Gene ID	1812
Other Names	D(1A) dopamine receptor; Dopamine D1 receptor
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Dopamine Receptor D1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DRD1 {ECO:0000303\|PubMed:33571432, ECO:0000312\|HGNC:HGNC:3020}
Function	G protein-coupled receptor for dopamine, a catecholamine neurotransmitter hormone that functions as the brain's 'reward chemical', driving motivation, reinforcement learning and pleasure (PubMed:11500503, PubMed:33571431, PubMed:33571432, PubMed:33750903, PubMed:34083522, PubMed:35676276, PubMed:35687690, PubMed:37221270). Dopamine binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:33571431, PubMed:33571432, PubMed:33750903, PubMed:34083522, PubMed:35676276, PubMed:35687690). Dopamine receptors can be classified in two categories: (1) DRD1 (together with DRD5) is a D1-type receptor and is coupled to G(s) G proteins, mediating activation of adenylate cyclase activity, while D2-type receptors (DRD2, DRD3 and DRD4) are coupled to G(i)/G(o) G proteins and promote inhibition of adenylate cyclase activity (PubMed:33571431, PubMed:33571432, PubMed:33750903, PubMed:34083522, PubMed:35676276, PubMed:35687690, PubMed:7782330). D1- and D2-type dopamine receptors have antagonistic effects, particularly in the brain's striatum, where they govern voluntary movement and reward: D1-type receptors generally stimulate neural activity and promotes action, while D2-type receptors tend to inhibit neural activity and suppress movement (By similarity). DRD1 acts as an essential regulator of reward, motor activity and cognition in the central nervous system and play beneficial roles in peripheral tissues including inhibiting inflammatory reactions and maintaining cardiovascular and kidney homeostasis (PubMed:33571431, PubMed:33571432). DRD1 and DRD2 are the most abundant dopamine receptors in the central nervous system, especially in the basal ganglia and the prefrontal cortex (PubMed:33571431, PubMed:33571432). DRD1 can also couple with G(q) by forming a heteromer with the ghrelin receptor GHSR in hippocampal neurons independently of ghrelin (By similarity). Following DRD1 activation by dopamine, GHSR acts as a coreceptor promoting DRD1 coupling to G(q) at the expense of G(s), initiating hippocampal synaptic plasticity involved in memory and learning (By similarity). Forms heterotetramers with DRD3 to potentiate beta-arrestin recruitment and mediate locomotor activity (By similarity). Also acts as a receptor for D-lysergic (LSD) psychedelic drug (PubMed:39094559).
Cellular Location	Postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendritic spine membrane {ECO:0000250\|UniProtKB:Q61616}; Multi-pass membrane protein. Cell projection, cilium membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P18901}; Multi-pass membrane protein Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. {ECO:0000250\|UniProtKB:P18901}
Tissue Location	Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Dearry A.,et al.Nature 347:72-76(1990).
Zhou Q.-Y.,et al.Nature 347:76-80(1990).
Sunahara R.K.,et al.Nature 347:80-83(1990).
Ohara K.,et al.Neuropsychopharmacology 8:131-135(1993).
Suwa M.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.

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Cat# AP51944

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Anti-Dopamine Receptor D1 Antibody

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