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Anti-ALPI Antibody

Western blot analysis of ALPI expression in HepG2 (A), Raw264.7 (B), PC12 (C) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 67 kD)
Immunofluorescent analysis of ALPI staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P09923
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56812 Da

Additional Information
Gene ID	248
Other Names	Intestinal-type alkaline phosphatase; IAP; Intestinal alkaline phosphatase
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ALPI. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ALPI
Function	Alkaline phosphatase that can hydrolyze various phosphate compounds.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P15693}; Lipid-anchor, GPI-anchor {ECO:0000250\|UniProtKB:P15693}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Berger J.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:695-698(1987).
Henthorn P.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:1234-1238(1987).
Henthorn P.S.,et al.J. Biol. Chem. 263:12011-12019(1988).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).

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¥ 1,250.00

Cat# AP51973

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100 µl

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Anti-ALPI Antibody

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