> 首页 > 产品 > 一抗 > 精选抗体 > G蛋白偶联受体抗体（GPCR） > Dopamine D5 receptor Rabbit pAb

Dopamine D5 receptor Rabbit pAb

Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- DRD5 antibody(AP52162), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (AP52162, 1 µg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of AP52162 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Sample:
Skin(Mouse) Lysate at 40 ug
Skin(Rat) Lysate at 40 ug
Primary: Anti-Dopamine D5 receptor (AP52162) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P25115
Reactivity	Rat, Mouse
Predicted	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	52831 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from rat DRD5
Epitope Specificity	251-350/475
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane; Multi-pass membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. This receptor is neuron specific, localized primarily within limbic regions of the brain. Defects in DRD5 are a cause of blepharospasm and may be a cause of schizophrenia.

Additional Information
Gene ID	25195
Other Names	D(1B) dopamine receptor, D(5) dopamine receptor, Dopamine D5 receptor, Drd5
Dilution	WB=1:500-2000,Flow-Cyt=1 µg /test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Drd5
Function	Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Brain, in the lateral mammillary nuclei, the anterior pretectal nuclei, and several layers of the hippocampus

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

REFERENCES

Sunahara R.K.,et al.Nature 350:614-619(1991).
Grandy D.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:9175-9179(1991).
Weinshank R.L.,et al.J. Biol. Chem. 266:22427-22435(1991).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP52162

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

reduce add

Availability: 3-5 days

询价

Dopamine D5 receptor Rabbit pAb