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Anti-PP15 Antibody

Western blot analysis of PP15 expression in HepG2 (A), MCF7 (B), CT26 (C), C6 (D) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14; 28; 42 kD)
Immunohistochemical analysis of PP15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PP15 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blot analysis of PP15 expression in MOLT4 (A) whole cell lysates.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P61970
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	14478 Da

Additional Information
Gene ID	10204
Other Names	NTF2; Nuclear transport factor 2; NTF-2; Placental protein 15; PP15
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PP15. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NUTF2 (HGNC:13722)
Function	Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.
Cellular Location	Cytoplasm, cytosol. Nucleus outer membrane {ECO:0000250\|UniProtKB:P61972}. Nucleus, nuclear pore complex {ECO:0000250\|UniProtKB:P61972}. Nucleus inner membrane {ECO:0000250\|UniProtKB:P61972}. Nucleus, nucleoplasm. Note=At steady state it is essentially nucleoplasmic, enriched in nucleoplasmic foci

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Grundmann U.,et al.Nucleic Acids Res. 16:4721-4721(1988).
Paschal B.M.,et al.J. Cell Biol. 129:925-937(1995).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP53312

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Anti-PP15 Antibody

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