CDT1/DUP Rabbit pAb

Western blot detection of CDT1 in K562,Jurkat cell lysates using CDT1 Rabbit pAb(1:1000 diluted).Predicted band size:60KDa.Observed band size:65KDa.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC, IP
Primary Accession	Q9H211
Host	Rabbit
Clonality	Polyclonal Antibody
Calculated MW	60390 Da

Additional Information
Gene ID	81620
Other Names	DNA replication factor Cdt1, Double parked homolog, DUP, CDT1 {ECO:0000312\|EMBL:AAG45181.1}
Dilution	WB~~1:1000 IHC~~1:100~500 FC~~1:10~50 IP~~N/A
Format	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	CDT1 {ECO:0000312\|EMBL:AAG45181.1}
Function	Required for both DNA replication and mitosis (PubMed:11125146, PubMed:14993212, PubMed:21856198, PubMed:22581055, PubMed:26842564). DNA replication licensing factor, required for pre- replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC) (PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity).
Cellular Location	Nucleus. Chromosome, centromere, kinetochore. Note=Transiently localizes to kinetochores during prometaphase and metaphase