Anti-MAGEA3/6 Antibody

Western blot analysis of MAGEA3/6 expression in mouse muscle (A), rat kidney (B) whole cell lysates.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P43360
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	34891 Da

Additional Information
Gene ID	4105
Other Names	MAGE6; Melanoma-associated antigen 6; Cancer/testis antigen 1.6; CT1.6; MAGE-6 antigen; MAGE3B antigen
Target/Specificity	Recognizes endogenous levels of MAGEA6 protein.
Dilution	WB~~1/500 - 1/1000
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	MAGEA6 {ECO:0000303\|PubMed:31267705, ECO:0000312\|HGNC:HGNC:6804}
Function	Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy (PubMed:17942928, PubMed:20864041, PubMed:31267705). May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:17942928, PubMed:20864041). May play a role in tumor transformation or aspects of tumor progression (PubMed:17942928, PubMed:20864041). In vitro promotes cell viability in melanoma cell lines (PubMed:17942928).
Tissue Location	Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes