Anti-UEVLD Antibody

Western blot analysis of UEVLD expression in Hela (A), Jurkat (B), H9C2 (C), AML12 (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
Immunohistochemical analysis of UEVLD staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of UEVLD staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8IX04
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	52264 Da

Additional Information
Gene ID	55293
Other Names	UEV3; Ubiquitin-conjugating enzyme E2 variant 3; UEV-3; EV and lactate/malate dehydrogenase domain-containing protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UEVLD. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1/50 - 1/100
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	UEVLD {ECO:0000312\|EMBL:AAH64566.1}
Function	Possible negative regulator of polyubiquitination.
Tissue Location	Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.