Anti-GPR151 Antibody

Western blot analysis of GPR151 expression in rat lung (A) whole cell lysates.
Immunofluorescent analysis of GPR151 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	Q8TDV0
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46637 Da

Additional Information
Gene ID	134391
Other Names	PGR7; Probable G-protein coupled receptor 151; G-protein coupled receptor PGR7; GPCR-2037
Target/Specificity	Recognizes endogenous levels of GPR151 protein.
Dilution	WB~~1/500 - 1/1000 IF~~1/50 - 1/200
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	GPR151
Synonyms	GALR4, GALRL {ECO:0000303\|PubMed:1511101
Function	Proton-sensing G-protein coupled receptor.
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	High expression in the spinal cord.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.