Anti-Collagen-pan Antibody

Western blot analysis of Collagen-pan expression in NIHC3T3 (A) whole cell lysates. (Predicted band size: 138; 141; 183 kD; Observed band size: 220 kD)
Immunohistochemical analysis of Collagen-pan staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Collagen-pan staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P02452
Other Accession	P02458, P02461, P20908
Reactivity	Human, Mouse, Rat, Chicken, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	138911 Da

Additional Information
Gene ID	1277
Other Names	Collagen alpha-1(I) chain; Alpha-1 type I collagen; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(III) chain
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen-pan. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	COL1A1
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Cellular Location	Secreted, extracellular space, extracellular matrix {ECO:0000255\|PROSITE-ProRule:PRU00793}
Tissue Location	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.