Serine racemase Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Serine racemase (AP54272) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation:Anti-Serine racemase Polyclonal Antibody, Unconjugated(AP54272) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample: lovo (human)cell Lysate at 40 ug
Primary: Anti- Serine racemase (AP54272) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q9GZT4
Reactivity	Rat, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	36566 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Serine racemase
Epitope Specificity	1-100/430
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the serine/threonine dehydratase family.
SUBUNIT	Homodimer.
Post-translational modifications	S-nitrosylated, leading to decrease the enzyme activity.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Known to be prominent in bacteria, D amino acids were generally thought to be absent in mammals. D-serine has since been found in high levels in the mammalian brain and in various mammalian fluids. D-serine activates N-methyl-D-aspartate (NMDA) receptors--molecules with important roles in learning, brain growth and brain cell death. Serine racemase is the enzyme catalyzing the formation of D-serine from L-serine. Serine racemase is a member of the family of pyridoxal-5’ phosphate-dependent enzymes and is localized to glial cells in rat brain.

Additional Information
Gene ID	63826
Other Names	Serine racemase, 5.1.1.18, D-serine ammonia-lyase, D-serine dehydratase, 4.3.1.18, SRR
Target/Specificity	Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	SRR
Function	Catalyzes the synthesis of D-serine from L-serine (PubMed:20106978, PubMed:23391306, PubMed:29277459). D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine (By similarity).
Tissue Location	Expressed in the cerebellum, hippocampus, dorsolateral prefrontal cortex, and in motor neurons and glial cells of the lumbar spinal cord (at protein level) (PubMed:17880399, PubMed:24138986). Increased in the dorsolateral prefrontal cortex of schizophrenic patients (at protein level) (PubMed:17880399). Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei (PubMed:15193426). Expressed in heart, skeletal muscle, kidney, and liver (PubMed:15193426)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.