Progestin Receptor Beta Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample:
Lane 1: Hippocampus (Mouse) Lysate at 40 ug
Lane 2: Medulld (Mouse) Lysate at 40 ug
Lane 3: Hippocampus (Rat) Lysate at 40 ug
Lane 4: Medulld (Rat) Lysate at 40 ug
Primary:
Anti-Progestin Receptor Beta (AP54490) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (Dog skin tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Progestin Receptor Beta) Polyclonal Antibody, Unconjugated (AP54490) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q8TEZ7
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40464 Da

Additional Information
Gene ID	85315
Other Names	Membrane progestin receptor beta, mPR beta, Lysosomal membrane protein in brain 1, Membrane progesterone P4 receptor beta, Membrane progesterone receptor beta, Progesterone and adipoQ receptor family member 8, Progestin and adipoQ receptor family member 8, Progestin and adipoQ receptor family member VIII, PAQR8 (HGNC:15708)
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	PAQR8 (HGNC:15708)
Function	Plasma membrane progesterone (P4) receptor coupled to G proteins (PubMed:23763432). Seems to act through a G(i) mediated pathway (PubMed:23763432). May be involved in oocyte maturation (By similarity). Also binds dehydroepiandrosterone (DHEA), pregnanolone, pregnenolone and allopregnanolone (PubMed:23161870).
Cellular Location	Cell membrane; Multi-pass membrane protein. Note=Colocalizes with a lysosomal protein CTSD/cathepsin D.
Tissue Location	Highly expressed in the hypothalamus (PubMed:23161870). Also expressed in spinal cord, kidney and testis